Complete plants with cladode, crown and root systems were developed in hormone free. This site is like a library, you could find million book. The roots are said to be tonic and diuretic and galactgogue, the drug has ulcer healing effect probably via strenthening the mucosal resistance or cytoprotection. Asparagus racemosusethnopharmacological evaluation and. Phytochemical attributes of endemic endangered primrose.
P3007 development of a super spud through repeated cycles of marker free transformation. A induction of calli on shoot tips and nodal bud explants on ms medium supplemented with 4 mg l1 bap and 0. Protocols for in vitro cultures and secondary metabolite. The succulent tuberous roots are 30100 cm long and 12 cm thick in bunch attached at the stem base. Sterile cultures were obtained when the explants were treated with 0. By in vitro propagation of asparagus, root initiation is difficult. Asparagus racemosus is an herb used in ayurveda medicine. In vitro plantlet regeneration in asparagus racemosus. Little work has been undertaken on in vitro culture in asparagus racemosus as compared to that of a. Efficient plant regeneration of asparagus by inducing normal.
Asparagus racemosus leaf extract inhibits growth of uok. Oct 14, 2015 in vitro seed germination and micropropagation of primrose primula heterochroma stapf. Asparagus racemosus is a commercially important medicinal plant, traditionally used for combating gynecological problems in india. In vitro propagation of wild asparagus adscendens roxb. Asparagus racemosus is one of the important medicinal plants found in india, china and other parts of the world. Micropropagation and phytochemical analysis of asparagus racemosus was investigated in the present study. The demand for asparagus racemosus in 20012002 was 10,924. Jan 11, 2011 a successful micropropagation system was developed for four different medicinal maesa species. Micropropagation of asparagus densiflorus via axillary. This plant is known to produce steroidal saponins called shatavarins. The leaves and the tuberous roots of asparagus are medically important in several diseases. Pdf asparagus officinalis is most extensively studied species within the genus asparagus, which is well known as garden asparagus. Asparagus racemosus shatavari is a plant with immense medicinal values.
The plant kingdom represents an enormous reservoir of. World health organization who estimates that the holistic system is gaining more popularity. Leaves contain 12 withanolides, alkaloids, glycosides, glucose and free amino acids. But its what is below ground that has captured and held the reverence of ayurveda for the past few millennia. Micropropagation of asparagus racemosus shatavari asparagus racemosus shatavari is a plant with immense medicinal values.
Asparagus racemosus is the one most commonly used in traditional medicine 1. Asparagus racemosus satavar, shatavari, or shatamull, shatawari is a species of asparagus common throughout india and the himalayas. In the present study, somatic embryogenesis via zygotic embryos was studied in asparagus racemosus willd. This study was initiated to optimize the conditions for production of shatavarins in cell cultures of a. It is reported to be recalcitrant to tissue culture practices. Ashtawarga plants suffering a triple standardization. Asparagus adscendens, in vitro regeneration, multiple shoots, nodal explant. Family asparagaceae asparagus racemosus, commonly called satavar, shatavari, or shatamull, is native to the himalayas in india. It grows 12 m 3 ft 3 in6 ft 7 in tall and prefers to take root in gravelly, rocky soils high up in piedmont plains, at 1,3001,400 m 4,3004,600 ft elevation. In some cases, tissue and cell culture is also used for the. Mohommad shahid department of botany, davpg college, dehradun. Asparagus racemosus a medicinally important plant can be propagated through tissue culture technique which save lots of time and money.
It is good source of folic acid, potassium, dietary fiber. According to the website of american diabetes association type 2 diabetes is the most common form of diabetes, in type 2 diabetes, either the body does not produce enough insulin or the cells ignore. In indian system of medicine asparagus racemosus is an important medicinal plant and its root paste or root juice has been used in various ailments and as health tonic 1,2. This study was carried out to establish an efficient in vitro propagation protocol. The present study on micropropagation of asparagus racemosus through nodal explants showed better result in bap 2 mgl along with iaa 0. The genus is considered to be medicinally important because of the presence of steroidal saponins and sapogenins in various parts of the plant. Safed musli ashwagandha shatavari churna hindi pdf herbal safedmusliashwagandhashat avarichurnahindi pdf. Since the callus quality plays an important role in plantlet development, therefore, compact embryogenic callus was selected for further embryogenesis. Multiple shoots were induced through both axillary bud formation and adventitious shoot regeneration from leaf explants. Somatic embryogenesis is one of the most popular in vitro regeneration methods for mass micropropagation. Rapid in vitro protocol for high multiple shoot induction. According to the website of american diabetes association type 2 diabetes is the most common form of diabetes, in type 2 diabetes, either the body does not. Shatavarin production from in vitro cultures of asparagus. They reported that naa singly showed an increase in callus induction from nodes, internodes and shoot tips of asparagus racemosus.
Direct in vitro propagation of asparagus adscendens. Asparagus officinalis as a valuable medicinal plant has a low multiplication rate using the conventional methods. Asparagus racemosus is a well known ayurvedic rasayana which prevent ageing, increase longevity, impart immunity, improve mental function, vigor and add. Various plant parts are consumed in a variety of ways, viz. Free aminoacids in the roots include aspartic acid, glycine, tyrosine, alanine, proline, tryptophan, glutamic acid and cystine. The asparagus racemosus shatavari or sanspayein is most commonly distributed species in tropical and subtropical regions of india. Induction of somatic embryos in cultures of asparagus. Its medicinal usage has been reported in the indian and british pharmacopoeias and in traditional systems of medicine such as ayurveda, unani, and siddha.
Asparagus racemosus is one of the important medicinal plants extensively used by the traditional practioners in india for its medicinal value. Rapid in vitro micro propagation of asparagus racemosus willd. Study of anatomical biomarkers for the standardization of. In vitro regeneration with callus development of asparagus.
The present study on micropropagation of asparagus racemosus through nodal explants showed better result in bap 2 mgl along. Shatavari is in the wild asparagus family, hence its botanical name asparagus racemosus. In vitro propagation of a high value medicinal plant. Overview information asparagus racemosus is a plant used in traditional indian medicine ayurveda. Active ingredients of asparagus racemosus are believed to be four saponins shatavarin i to iv. Plants are valuable sources of a vast array of chemical compounds like alkaloids, flavonoids, pigments, antimicrobials, pharmaceutical, etc. Study of phytohormones effect on micropropagation and shoot induction in asparagus racemosus int j cur res rev, nov 2012 vol 04 22 page 48 in case of reproductive disease. Shatavarin iv is a glycoside of sarasapogenin having two molecules of rhamnose and one molecule of glucose 2, 3. The majority of the compounds were produced by plants during different stages of cell, tissues and organ differentiation. This plant possesses a wide range of secondary metabolites inclusive of steroids, alkaloids, dihydrophenanthrene. In asparagus racemosus, there is an almost 100% mark up in price from the collector level to the user tewari, 2000. Asparagus racemosus, traditionally known as shatavari means who medicines, lists asparagus racemosus as part of thomson m.
Introduction asparagus racemosus have numerous medicinal usages which have been reported in the indian and british pharmacopoeias and in indigenous systems of medicine. Survival of cultured cells and somatic embryo of asparagus pdf. Hisato kunitake and masahiro 201224 from the epidermal cells of explants or indirectly via intervening callus 4. Translated as having one hundred roots and also referred to as meaning having one hundred husbands, 1, 2 shatavaris name gives reference to its traditional use as a. Micropropagation of asparagus densiflorus via axillary shoots, indirect organogenesis and somatic embryogenesis anna pindel a. P3026 the molecular events during the induction of shoot regeneration in plant tissue culture. Rooting was achieved with half strength ms basal medium plus iba. In wiley online library other applications of plant tissue culture which are not necessarily strictly propagation. Asparagus racemosus is a valuable source of antioxidant and has significant cytotoxic activity hence could eliminate many diseases related to free radical. Plant tissue culture is the process by which parts of plant can be grown in vitro condition in a sterile culture medium. The explants were cultured on murashige and skoog ms medium supplemented with 6benzyladenine ba, thidiazuron tdz andor. Berries contain a milk coagulating enzyme, two esterases, free amino acids, fatty oil, essential oil and alkaloids. Conservation of medicinal plant asparagus racemosus.
Supplementing asparagus racemosus will aid digestion, since the plant has antiulcer effects. It is an extensively scandent spinous, much branched undershrub. The species is hermaphrodite has both male and female organs and is pollinated by bees. It is an undershrub climber with extensively branched woody stems, growing up to 2m in height. The present study on micropropagation of asparagus racemosus through nodal. In vitro propagation of spine gourd momordica dioica roxb. Investigation of cytotoxicity and invitro antioxidant. Moshe reuveni, aro, volcani center, dalia evenor, and mike timko. Asparagus racemosus, a climbing ayurvedic plant, is known for its numerous activities such as hyperlipidemia, hypertension, angina, dysmenorrhea, anxiety disorders, benign prostatic hyperplasia bph, leucorrhoea and urinary tract infections. Asparagus racemosus, pharmacognosy, pharmacology, phytochemistry, shatavarins 1. C regenerated shoots on sem with 1 mg l1 bap and 0. In vitro micro propagation of soybean glycine max bari5. The therapeutic applications of this plant have been reported in indian and british pharmacopoeias and in traditional system of medicine, such as ayurveda, unani and siddha.
Asparagus racemosus supplement health benefits, dosage. Roots numerous fusiform, succulent and tuberous with a diameter of 0. Ayurveda is one of the oldest known holistic health care systems recommending diverse medicinal uses of plants for prevention and cure of diseases and illness. Neet biology strategies for enhancement in food production. Micropropagation and phytochemical analysis of asparagus. The majority of plants used by the pharmaceutical industry come from wild sources, endangering the natural population of the species.
Asparagus racemosus due to its slow growth faces lots of problem which can be solved through tissue culture. Out of the 22 species of asparagus recorded in india. Rhizogenesis did not occur in individual shoots, upon their transfer to hormone free. The plants are being overharvested, so this species faces a real danger of becoming. Randomized controlled trial of asparagus racemosus shatavari as a lactogogue in lactational inadequacy. The multiple uses of this species have increased its commercial demand resulting in overexploitation.
Regeneration of asparagus racemosus by shoot apex and. Three different media representing the whole micropropagation protocol of asparagus are described. In vitro cellular and developmental biology plant bioone. Pdf asparagus racemosus commonly known as shatavari in. Ms, full strength of ms basal medium supplemented with 3 and 6% sucrose and white root culture medium. Propagation of asparagus racemosus through tissue culture. Asparagus racemosus wild locally known as satawar belongs to the family liliaceae. The plant occurs throughout india up to 1500 meters elevation. All books are in clear copy here, and all files are secure so dont worry about it. Asparagus racemosus is a perennial climber climbs upto m high. Jan 17, 2017 now a day ayurvedic products are suffering from a serious problem of adulteration with addition of low grade, spoiled, inferior, spurious drugs, and useless parts of same or different plant, harmful substances or totally different drug either free from or inferior in therapeutic and chemical properties from original drug which do not confirm.
This plant possesses a wide range of secondary metabolites inclusive of steroids, alkaloids, dihydrophenanthrene derivatives, flavonoids, furan. Pdf micropropagation of asparagus by in vitro shoot culture. Shatavari, micropropagation, tissue culture, in vitro culture. Asparagus racemosus in terms of qualitative and quantitative parameters of milk, in g 1 was control group, in which the animals receive experimental feed without any other feed supplements, whereas the animals of experimental group 2 g 2, group 3 g 3 and group 4 g 4 were fed a similar. Micropropagation of asparagus racemosus shatavari abstract. It will also aid the immune system when antibodies are fighting off a threat. Murashige and skoogs medium with 2, 4d and kinetin induced callus in the shoot segments of asparagus racemosus. The multiple uses of this species have increased its commercial demand, resulting in overexploitation. Pdf micropropagation and phytochemical analysis of. Liliaceae, commonly known as satawari hindi is a perennial shrub, with a tuberous rootstock, stems covered with recurved spines, linear leaves arranged in a tuft, white flowers and sweetscented appears in october. Pdf micropropagation and phytochemical analysis of asparagus. In vitro propagation of four saponin producing maesa species.
Radical scavenging, dpph, phenolic, flavonoid, cupric, reducing power. Sep 23, 2011 asparagus racemosus is an important monocot medicinal plant that is in great demand for its steroidal saponins called shatavarins. Notes, study material, practice question and answers, online tests,download pdf. Asparagus racemosus is usually propagated by planting the separated tuberous roots along with shoot apex. Asparagus racemosus is a perennial growing to 7 m 23ft. Effect of feeding shatavari asparagus racemosus root. Neet biology strategies for enhancement in food production prepare. Manjeshwar shrinath baliga, in foods and dietary supplements in the prevention and treatment of disease in older adults, 2015. Asparagus racemosus an overview sciencedirect topics. Request pdf in vitro propagation of a high value medicinal plant. It is a beautiful plant with wispy, graceful stems and branches, thin, needlelike leaves, and delicate flowers. Liliaceae is a pharmacologically acclaimed phytoestrogenic medicinal plant used for its immunomodulatory diwanay et al. Shatavari is a special female tonic because it helps women of all age groups to transit through natural. The present study on micropropagation of asparagus racemosus through.
Most of the cultures in hormone media induced callus in the beginning but ms hormone free media showed no response of any kind in callus induction with all the. The genus asparagus includes about 300 species around the world. Hair regrowth treatment provillus november 03, 2017. Micropropagation of ringal bamboo drepanostachyum falcatum nees keng f. Review plant biotechnology, 152,51611998 somatic embryogenesis and its application for breeding and micropropagation in asparagus asparagus oflicinalis l. The optimal medium for asparagus micropropagation described in this. The genus asparagus consisted of about 300 species around the world, out of which 22 species are recorded in india.
Regeneration of shoot buds and clonal multiplication of excised shoots through proliferation of nodal buds could be achieved by the use of iaa and bap in the medium. Leaf and underground stem explants produced maximum number of shoots four and 10 per explant, respectively on b5 medium supplemented with 4. Shatavari asparagus racemosus is a powerful therapeutic agent, which is of great importance in ayurveda. Micropropagation of asparagus racemosus shatavari semantic. In vitro plantlet regeneration in asparagus racemosus through shoot bud differentiation on nodal segmentsashwani kumar and neetu vijay. B adventitious shoot formation from surface of calli on ms with 2 mg l1 and 0.
Shatavari, or asparagus racemosus, has been used for centuries in ayurveda to support the reproductive system, particularly for females, and as a support for the digestive system, especially in cases of excess pitta. The plant with immense medicinal potential lakhwinder singh, antul kumar, anuj choudhary and gurwinder singh abstract the genus asparagus belonging to the family liliaceae. M in the medium completely inhibited the growth and. Asparagus leaf extract inhibits growth of renal cell cancer cells asian pac j cancer prev, 15 5, 19371941 introduction asparagus racemosus willd.
1397 152 615 1262 316 827 1266 321 1596 1485 779 1202 1257 1577 521 389 117 1409 564 773 748 16 1442 1356 120 1058 272 683 866 580 262 1466 1320